Magnetic FFPE Tissue DNA Extraction Kit

 

EmerTher® Magnetic FFPE Tissue DNA Extraction Kit is used for extracting DNA from formaldehyde fixed-paraffin embedded (FFPE) tissue. 

 

The Kit contains superparamagnetic nanoparticles which are bound to nucleic acids and an efficient extraction system. The magnetic beads are coated through a unique process, enabling strong binding with nucleic acids and easy elution. 

 

The experimental procedure is simple and efficient: 1) following pretreatment, add sample to a lysis-binding buffer to enable cell lysis, DNA release and DNA binding to magnetic beads in one step; 2) apply magnetic force enabling easy wash of the beads with buffers; 3) elute DNA from the beads using elution buffer. The extraction procedure is fully compatible with automation. 

 

Purified DNA can be directly used in a variety of downstream applications, including PCR, gene sequencing, etc. 

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Features

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• Eliminate the need to use xylene, a highly toxic solvent commonly used for dissolving paraffin.

• Convenient: No tissue grinding is necessary.

• Efficient decoupling effect: Effectively remove cross-linking between nucleic acids and proteins formed during formalin fixation, increasing the sensitivity and consistency of subsequent tests.

• Retain large DNA pieces, facilitating subsequent tests.

• Can be used with EmerTher magnetic FFPE tissue RNA extraction kit to extract DNA and RNA from one sample. 

• Automation: compatible with a variety of automatic magnetic bead processors; prefilled plates are available.