EmerTher® Magnetic FFPE Tissue RNA Extraction Kit is used for extracting RNA from formaldehyde fixed-paraffin embedded (FFPE) tissue.
The Kit contains superparamagnetic nanoparticles which are bound to nucleic acids and an efficient extraction system. The magnetic beads are coated through a unique process, enabling strong binding with nucleic acids and easy elution.
The experimental procedure is simple and efficient: 1) following pretreatment, add sample to a lysis-binding buffer to enable cell lysis, RNA release and RNA binding to magnetic beads in one step; 2) apply magnetic force enabling easy wash of the beads with buffers; 3) elute RNA from the beads using an elution solution. The extraction procedure is fully compatible with automation.
Purified RNA can be directly used in a variety of downstream experiments, including PCR, gene sequencing, etc.
Eliminate the need for xylene, a toxic solvent commonly used for dissolving paraffin
Eliminate the use of toxic chloroform and phenol
Convenient: No tissue grinding is necessary
Effectively remove cross-linking between nucleic acids and proteins formed during formalin fixation, preventing inhibition of reverse transcription of the extracted RNA due to cross-linkage and increasing the sensitivity and consistency of subsequent tests
Retain large RNA pieces, facilitating subsequent testing
Automation: compatible with a variety of automatic magnetic bead processors; pre-packed plates are available
Magnetic FFPE-Tissue RNA Extraction Kit
Specifications：pre-filled plates (96 preps); bottles (20 and 100 preps)
Components：paraffin sample solution, paraffin digestion solutions, proteinase K, magnetic beads, lysis-binding buffer, wash solution I, wash solution II, wash solution III, elution solution.
- Stored at room temp. Proteinase K needs to be stored at -20°C. Shelf time: 1 year.