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1. Description

EmerTher® Magnetic FFPE Tissue RNA Extraction Kit is used for extracting RNA from formaldehyde fixed-paraffin embedded (FFPE) tissue. 

The Kit contains superparamagnetic nanoparticles which are bound to nucleic acids and an efficient extraction system. The magnetic beads are coated through a unique process, enabling strong binding with nucleic acids and easy elution. 

The experimental procedure is simple and efficient: 1) following pretreatment, add sample to a lysis-binding buffer to enable cell lysis, RNA release and RNA binding to magnetic beads in one step; 2) apply magnetic force enabling easy wash of the beads with buffers; 3) elute RNA from the beads using an elution solution. The extraction procedure is fully compatible with automation. 

Purified RNA can be directly used in a variety of downstream experiments, including PCR, gene sequencing, etc. 


2. Features

Eliminate the need for xylene, a toxic solvent commonly used for dissolving paraffin

Eliminate the use of toxic chloroform and phenol

Convenient: No tissue grinding is necessary

Effectively remove cross-linking between nucleic acids and proteins formed during formalin fixation, preventing inhibition of reverse transcription of the extracted RNA due to cross-linkage and increasing the sensitivity and consistency of subsequent tests

Retain large RNA pieces, facilitating subsequent testing

Automation: compatible with a variety of automatic magnetic bead processors; pre-packed plates are available

Magnetic FFPE-Tissue RNA Extraction Kit

  • Specifications:pre-filled plates (96 preps); bottles (20 and 100 preps)
    Components:paraffin sample solution, paraffin digestion solutions, proteinase K, magnetic beads, lysis-binding buffer, wash solution I, wash solution II, wash solution III, elution solution.  

    • Stored at room temp. Proteinase K needs to be stored at -20°C. Shelf time: 1 year.
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