EmerTher® Magnetic FFPE Tissue DNA Extraction Kit is used for extracting DNA from formaldehyde fixed-paraffin embedded (FFPE) tissue.
The Kit contains superparamagnetic nanoparticles which are bound to nucleic acids and an efficient extraction system. The magnetic beads are coated through a unique process, enabling strong binding with nucleic acids and easy elution.
The experimental procedure is simple and efficient: 1) following pretreatment, add sample to a lysis-binding buffer to enable cell lysis, DNA release and DNA binding to magnetic beads in one step; 2) apply magnetic force enabling easy wash of the beads with buffers; 3) elute DNA from the beads using elution buffer. The extraction procedure is fully compatible with automation.
Purified DNA can be directly used in a variety of downstream applications, including PCR, gene sequencing, etc.
Eliminate the need to use xylene, a highly toxic solvent commonly used for dissolving paraffin.
Convenient: No tissue grinding is necessary.
Efficient decoupling effect: Effectively remove cross-linking between nucleic acids and proteins formed during formalin fixation, increasing the sensitivity and consistency of subsequent tests.
Retain large DNA pieces, facilitating subsequent tests.
Can be used with EmerTher magnetic FFPE tissue RNA extraction kit to extract DNA and RNA from one sample.
Automation: compatible with a variety of automatic magnetic bead processors; prefilled plates are available.
Magnetic FFPE-Tissue DNA Extraction Kit
Specifications：pre-filled plates (96 preps); bottles (20 and 100 preps)
Components：paraffin sample solution, paraffin digestion solutions, proteinase K, magnetic beads, lysis-binding buffer, wash solution I, wash solution II, elution solution.
- Stored at room temp. Proteinase K needs to be stored at -20°C. Shelf time: 1.5 years.