Proteins as Biomarkers:
We’re Supporting Various Antibody-based Immunoassays
Reverse Phase Protein Array (RPPA)High-Throughput
Functional Proteomics for Discovery and Precision Medicine• High-throughput • Ultra-sensitive• Functional insights • Minimal sample (µg)TRUSTED PARTNER ACCELERATING DRUGDISCOVERY & TRANSLATIONAL DEVELOPMENTTRUSTED PARTNER ACCELERATING DRUGDISCOVERY & TRANSLATIONAL DEVELOPMENTIntegration of RPPA & nCounter TechnologyTranscriptomics + Proteomics from One Sample: A Complete View of BiologyProfile ~600 proteins and ~770 transcripts simultaneouslyUncover MoA and pathway activation from one sample
WHAT IS RPPA & WHY IT MATTERS
RPPA is a high-throughput, antibody-based proteomic array that quantitatively measuresprotein expression and post-translational modifications across thousands of samples. Unlikegenomic or transcriptomic methods, RPPA captures functional protein activity, providinginsights into signaling pathways, disease mechanisms, and treatment responses.
High sensitivity, minimal sample input, scalable, and actionable results
High multiplexity: Enables the detection and quantification
of hundreds of distinct proteins, including their expression
levels and modification status, using only 40 µg of cell
lysates (currently covering ~ 600 proteins from various
signaling pathways).
Cost-effective: $1,000 to 3,000 per sample, depending on
the quantity of samples and sample types.
High sensitivity: Exhibits greater sensitivity than ELISA
while requiring minimal sample consumption.
Truly quantitative: RPPA enables precise measurement of
protein expression and post-translational modifications,
calibrated with recombinant proteins and phospho-peptide
standards.
Simplified: No direct labeling or labor-intensive depletion
or fractionation of sample lysates is required.
Versatile sample types: Various types of samples can be
investigated, including those from cell cultures, live animals
and patients (such as fine needle aspirates, laser capture
microdissection, FFPE samples), sorted cells, as well as
serum, plasma, saliva, urine, etc.
FPPA (Forward Phase Protein Array) vs. RPPA (Reverse Phase Protein Array)
